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Adaptive illumination reduces photobleaching in structured illumination microscopy

Photobleaching is a major factor limiting the observation time in fluorescence microscopy. We achieve photobleaching reduction in structured illumination microscopy (SIM) by locally adjusting the illumination intensities according to the sample. Adaptive SIM is enabled by a digital micro-mirror devi...

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Détails bibliographiques
Publié dans:Biomed Opt Express
Auteurs principaux: Chakrova, Nadya, Canton, Alicia Soler, Danelon, Christophe, Stallinga, Sjoerd, Rieger, Bernd
Format: Artigo
Langue:Inglês
Publié: Optical Society of America 2016
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5102519/
https://ncbi.nlm.nih.gov/pubmed/27867730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.7.004263
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