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Adaptive illumination reduces photobleaching in structured illumination microscopy

Photobleaching is a major factor limiting the observation time in fluorescence microscopy. We achieve photobleaching reduction in structured illumination microscopy (SIM) by locally adjusting the illumination intensities according to the sample. Adaptive SIM is enabled by a digital micro-mirror devi...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Chakrova, Nadya, Canton, Alicia Soler, Danelon, Christophe, Stallinga, Sjoerd, Rieger, Bernd
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5102519/
https://ncbi.nlm.nih.gov/pubmed/27867730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.7.004263
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