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Direct enzymatic synthesis of a deep-blue fluorescent noncanonical amino acid from azulene and serine

We report a simple, one-step enzymatic synthesis of the blue fluorescent noncanonical amino acid β-(1-azulenyl)-l-alanine (AzAla). Using an engineered tryptophan synthase β-subunit (TrpB), stereochemically pure AzAla can be synthesized at scale starting from commercially available azulene and l-seri...

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Detalles Bibliográficos
Publicado en:Chembiochem
Main Authors: Watkins, Ella J., Almhjell, Patrick J., Arnold, Frances H.
Formato: Artigo
Idioma:Inglês
Publicado: 2019
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6980211/
https://ncbi.nlm.nih.gov/pubmed/31513332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201900497
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