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Generation of biparatopic antibody through two-step targeting of fragment antibodies on antigen using SpyTag and SpyCatcher

Biparatopic fragment antibodies can overcome deficiencies in avidity of conventional antibody fragments. Here, we describe a technology for generating biparatopic antibodies through two-step targeting using a pair of polypeptides, SpyTag and SpyCatcher, that spontaneously react to form a covalent bo...

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Detalhes bibliográficos
Publicado no:Biotechnol Rep (Amst)
Main Authors: Akiba, Hiroki, Takayanagi, Kensuke, Kusano-Arai, Osamu, Iwanari, Hiroko, Hamakubo, Takao, Tsumoto, Kouhei
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6976922/
https://ncbi.nlm.nih.gov/pubmed/31993343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.btre.2020.e00418
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