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Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells

How genomic DNA is organized in the nucleus is a long-standing question. We describe a single-molecule bioimaging method utilizing super-localization precision coupled to fully quantitative image analysis tools, towards determining snapshots of parts of the 3D genome architecture of model eukaryote...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Methods
Hauptverfasser: Wollman, Adam J.M., Hedlund, Erik G., Shashkova, Sviatlana, Leake, Mark C.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Academic Press 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6971689/
https://ncbi.nlm.nih.gov/pubmed/31252059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2019.06.021
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