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The structure of the Thermococcus gammatolerans McrB N-terminal domain reveals a new mode of substrate recognition and specificity among McrB homologs

McrBC is a two-component, modification-dependent restriction system that cleaves foreign DNA-containing methylated cytosines. Previous crystallographic studies have shown that Escherichia coli McrB uses a base-flipping mechanism to recognize these modified substrates with high affinity. The side cha...

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Publicat a:J Biol Chem
Autors principals: Hosford, Christopher J., Bui, Anthony Q., Chappie, Joshua S.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6970917/
https://ncbi.nlm.nih.gov/pubmed/31822563
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.010188
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