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Use of a fluorescence assay to determine relative affinities of semisynthetic aminoglycosides to small RNAs representing bacterial and mitochondrial A sites

The off-target binding of aminoglycosides (AGs) to the A site of human mitochondrial ribosomes in addition to bacterial ribosomes causes ototoxicity and limits their potential as antibiotics. A fluorescence assay was employed to determine relative binding affinities of classical and improved AG comp...

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Detalhes bibliográficos
Publicado no:Bioorg Med Chem
Main Authors: Waduge, Prabuddha, Sati, Girish C., Crich, David, Chow, Christine S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6961810/
https://ncbi.nlm.nih.gov/pubmed/31610941
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmc.2019.115121
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