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Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids
Targeted genomic knock-ins are a valuable tool to probe gene function. However, knock-in methods involving homology-directed repair (HDR) can be laborious. Here, we adapt the mammalian CRISPaint [clustered regularly interspaced short palindromic repeat (CRISPR)-assisted insertion tagging] homology-i...
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| Pubblicato in: | Genetics |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Genetics Society of America
2020
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6944404/ https://ncbi.nlm.nih.gov/pubmed/31685521 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.119.302819 |
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