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Fast and Efficient Drosophila melanogaster Gene Knock-Ins Using MiMIC Transposons
Modern molecular genetics studies necessitate the manipulation of genes in their endogenous locus, but most of the current methodologies require an inefficient donor-dependent homologous recombination step to locally modify the genome. Here we describe a methodology to efficiently generate Drosophil...
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| Publicado no: | G3 (Bethesda) |
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| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4267933/ https://ncbi.nlm.nih.gov/pubmed/25298537 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.114.014803 |
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