Fast and Efficient Drosophila melanogaster Gene Knock-Ins Using MiMIC Transposons

Modern molecular genetics studies necessitate the manipulation of genes in their endogenous locus, but most of the current methodologies require an inefficient donor-dependent homologous recombination step to locally modify the genome. Here we describe a methodology to efficiently generate Drosophil...

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Dades bibliogràfiques
Publicat a:G3 (Bethesda)
Autors principals: Vilain, Sven, Vanhauwaert, Roeland, Maes, Ine, Schoovaerts, Nils, Zhou, Lujia, Soukup, Sandra, da Cunha, Raquel, Lauwers, Elsa, Fiers, Mark, Verstreken, Patrik
Format: Artigo
Idioma:Inglês
Publicat: Genetics Society of America 2014
Matèries:
Investigations
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4267933/
https://ncbi.nlm.nih.gov/pubmed/25298537
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.114.014803
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