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The Fluorescence Emission of the Apo-glucose Oxidase from Aspergillus niger as Probe to Estimate Glucose Concentrations
We developed a new method of glucose sensing using an inactive form of glucose oxidase from Aspergillus niger. Glucose oxidase was rendered inactive by removal of the FAD cofactor. The resulting apo-glucose oxidase still binds glucose as observed from a decrease in its intrinsic tryptophan fluoresce...
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| Udgivet i: | Biochem Biophys Res Commun |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1999
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6943336/ https://ncbi.nlm.nih.gov/pubmed/10491329 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1006/bbrc.1999.1330 |
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