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A Novel Fluorescence Competitive Assay for Glucose Determinations by Using a Thermostable Glucokinase from the Thermophilic Microorganism Bacillus stearothermophilus
We describe the use of a thermostable glucokinase in a novel competitive fluorescence assay for glucose. Glucokinase from Bacillus stearothermophilus (BSGK) was found to retain enzymatic activity in solution for over 20 days. The single cysteine residue in BSGK, which is near the active site, was la...
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| Publicado no: | Anal Biochem |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6905376/ https://ncbi.nlm.nih.gov/pubmed/11950213 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1006/abio.2001.5544 |
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