A carregar...
N-terminal fusion of the N-terminal domain of bacterial Enzyme I facilitates recombinant expression and purification of the human RNA demethylases FTO and Alkbh5
Various fusion tags are commonly employed to increase the heterologous expression and solubility of aggregation-prone proteins within Escherichia coli. Herein, we present a protocol for efficient recombinant expression and purification of the human RNA demethylases Alkbh5 and FTO. Our method incorpo...
Na minha lista:
| Publicado no: | Protein Expr Purif |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
|
| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6942671/ https://ncbi.nlm.nih.gov/pubmed/31740367 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2019.105540 |
| Tags: |
Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
|