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Tailoring Tryptophan Synthase TrpB for Selective Quaternary Carbon Bond Formation

We previously engineered the tryptophan synthase β-subunit (TrpB), which catalyzes the condensation of l-serine and indole to l-tryptophan, to synthesize a range of noncanonical amino acids from l-serine and indole derivatives or other nucleophiles. Here we employ directed evolution to engineer TrpB...

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Bibliographic Details
Published in:J Am Chem Soc
Main Authors: Dick, Markus, Sarai, Nicholas S., Martynowycz, Michael W., Gonen, Tamir, Arnold, Frances H.
Format: Artigo
Language:Inglês
Published: 2019
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6939453/
https://ncbi.nlm.nih.gov/pubmed/31747522
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.9b09864
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