FRET efficiency measurement in a molecular tension probe with a low-cost frequency-domain fluorescence lifetime imaging microscope

We demonstrate the possibility of measuring FRET efficiency with a low-cost frequency-domain fluorescence lifetime imaging microscope (FD-FLIM). The system utilizes single-frequency-modulated excitation, which enables the use of cost-effective laser sources and electronics, simplification of data ac...

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Detalhes bibliográficos
Publicado no:J Biomed Opt
Main Authors: Dumas, John-Paul, Jiang, James Y., Gates, Evan M., Hoffman, Brenton D., Pierce, Mark C., Boustany, Nada N.
Formato: Artigo
Idioma:Inglês
Publicado em: Society of Photo-Optical Instrumentation Engineers 2019
Assuntos:
Microscopy
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6935677/
https://ncbi.nlm.nih.gov/pubmed/31884745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.24.12.126501
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