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High-frequency random DNA insertions upon co-delivery of CRISPR-Cas9 ribonucleoprotein and selectable marker plasmid in rice

An important advantage of delivering CRISPR reagents into cells as a ribonucleoprotein (RNP) complex is the ability to edit genes without reagents being integrated into the genome. Transient presence of RNP molecules in cells can reduce undesirable off-target effects. One method for RNP delivery int...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Banakar, Raviraj, Eggenberger, Alan L., Lee, Keunsub, Wright, David A., Murugan, Karthik, Zarecor, Scott, Lawrence-Dill, Carolyn J., Sashital, Dipali G., Wang, Kan
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6934568/
https://ncbi.nlm.nih.gov/pubmed/31882637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-55681-y
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