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Normalization and variance stabilization of single-cell RNA-seq data using regularized negative binomial regression

Single-cell RNA-seq (scRNA-seq) data exhibits significant cell-to-cell variation due to technical factors, including the number of molecules detected in each cell, which can confound biological heterogeneity with technical effects. To address this, we present a modeling framework for the normalizati...

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Bibliografiske detaljer
Udgivet i:Genome Biol
Main Authors: Hafemeister, Christoph, Satija, Rahul
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6927181/
https://ncbi.nlm.nih.gov/pubmed/31870423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-019-1874-1
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