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Biochemical characterization of RNA-guided ribonuclease activities for CRISPR-Cas9 systems
The majority of bacteria and archaea rely on CRISPR-Cas systems for RNA-guided, adaptive immunity against mobile genetic elements. The Cas9 family of type II CRISPR-associated DNA endonucleases generates programmable double strand breaks in the CRISPR-complementary DNA targets flanked by the PAM mot...
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| Publicado en: | Methods |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2019
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6923617/ https://ncbi.nlm.nih.gov/pubmed/31228550 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2019.06.018 |
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