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PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System
Nowadays, CRISPR (clustered regularly interspaced short palindromic repeats) and the CRISPR-associated protein (Cas9) system play a major role in genome editing. To target the desired sequence of the genome successfully, guide RNA (gRNA) is indispensable for the CRISPR/Cas9 system. To express gRNA,...
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| Publié dans: | Bio Protoc |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Bio-Protocol
2021
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8292124/ https://ncbi.nlm.nih.gov/pubmed/34327279 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.4082 |
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