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PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System

Nowadays, CRISPR (clustered regularly interspaced short palindromic repeats) and the CRISPR-associated protein (Cas9) system play a major role in genome editing. To target the desired sequence of the genome successfully, guide RNA (gRNA) is indispensable for the CRISPR/Cas9 system. To express gRNA,...

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Détails bibliographiques
Publié dans:Bio Protoc
Auteurs principaux: Hassan, Naim, Easmin, Farhana, Ekino, Keisuke, Harashima, Satoshi
Format: Artigo
Langue:Inglês
Publié: Bio-Protocol 2021
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8292124/
https://ncbi.nlm.nih.gov/pubmed/34327279
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.4082
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