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Competition Assay for Measuring Deubiquitinating Enzyme Substrate Affinity
Assays of the affinity of a deubiquitinating enzyme for substrate, either through binding studies or determination of the Michaelis constant, K(M), can shed light on substrate selectivity and the effects of mutations on substrate interactions. The difficulty in generating sufficient quantities of ub...
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| Опубликовано в: : | Methods Mol Biol |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6917206/ https://ncbi.nlm.nih.gov/pubmed/30242703 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8706-1_5 |
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