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A Method for Assaying Deubiquitinating Enzymes
A general method for the assay of deubiquitinating enzymes was described in detail using (125)I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate. Since the tyrosine residue in the PESTc portion of the fusion protein was almost exclusively radioiodinated under a...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Biological Procedures Online
1998
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC140113/ https://ncbi.nlm.nih.gov/pubmed/12734599 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1251/bpo11 |
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