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A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resistance locus
Gene-drive systems in diploid organisms bias the inheritance of one allele over another. CRISPR-based gene-drive expresses a guide RNA (gRNA) into the genome at the site where the gRNA directs Cas9-mediated cleavage. In the presence of Cas9, the gRNA cassette and any linked cargo sequences are copie...
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| Publicado no: | Nat Commun |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6915771/ https://ncbi.nlm.nih.gov/pubmed/31844051 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-13649-6 |
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