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Extensive free-energy simulations identify water as the base in nucleotide addition by DNA polymerase

Transphosphorylation of nucleotide triphosphates is the central reaction in DNA replication by DNA polymerase as well as many other biological processes. Despite its importance, the microscopic chemical mechanism of transphosphorylation of nucleotide triphosphates is, in most cases, unknown. Here we...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Roston, Daniel, Demapan, Darren, Cui, Qiang
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6911213/
https://ncbi.nlm.nih.gov/pubmed/31757846
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1914613116
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