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Analysis of Phosphoryl-Transfer Enzymes with QM/MM Free Energy Simulations

We discuss the application of quantum mechanics/molecular mechanics (QM/MM) free energy simulations to the analysis of phosphoryl transfers catalyzed by two enzymes: alkaline phosphatase and myosin. We focus on the nature of the transition state and the issue of mechanochemical coupling, respectivel...

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Bibliografiske detaljer
Udgivet i:Methods Enzymol
Main Authors: Roston, Daniel, Lu, Xiya, Fang, Dong, Demapan, Darren, Cui, Qiang
Format: Artigo
Sprog:Inglês
Udgivet: 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6536301/
https://ncbi.nlm.nih.gov/pubmed/30149869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2018.05.005
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