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An arginase-based system for selection of transfected CHO cells without the use of toxic chemicals
Polyamines have essential roles in cell proliferation, DNA replication, transcription, and translation processes, with intracellular depletion of putrescine, spermidine, and spermine resulting in cellular growth arrest and eventual death. Serum-free media for CHO-K1 cells require putrescine suppleme...
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| Published in: | J Biol Chem |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Biochemistry and Molecular Biology
2019
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6901305/ https://ncbi.nlm.nih.gov/pubmed/31666335 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.011162 |
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