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An arginase-based system for selection of transfected CHO cells without the use of toxic chemicals

Polyamines have essential roles in cell proliferation, DNA replication, transcription, and translation processes, with intracellular depletion of putrescine, spermidine, and spermine resulting in cellular growth arrest and eventual death. Serum-free media for CHO-K1 cells require putrescine suppleme...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Capella Roca, Berta, Lao, Nga, Barron, Niall, Doolan, Padraig, Clynes, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6901305/
https://ncbi.nlm.nih.gov/pubmed/31666335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.011162
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