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Trapping and Electron Paramagnetic Resonance Characterization of the 5′dAdo(•) Radical in a Radical S-Adenosyl Methionine Enzyme Reaction with a Non-Native Substrate

[Image: see text] S-Adenosyl methionine (SAM) is employed as a [4Fe-4S]-bound cofactor in the superfamily of radical SAM (rSAM) enzymes, in which one-electron reduction of the [4Fe-4S]-SAM moiety leads to homolytic cleavage of the S-adenosyl methionine to generate the 5′-deoxyadenosyl radical (5′dAd...

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Publicat a:ACS Cent Sci
Autors principals: Sayler, Richard I., Stich, Troy A., Joshi, Sumedh, Cooper, Nicole, Shaw, Jared T., Begley, Tadhg P., Tantillo, Dean J., Britt, R. David
Format: Artigo
Idioma:Inglês
Publicat: American Chemical Society 2019
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6891858/
https://ncbi.nlm.nih.gov/pubmed/31807679
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acscentsci.9b00706
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