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Highly efficient methods for generating deletion mutations and F0 embryos that lack gene function in zebrafish
Inconsistent activity limits use of CRISPR/Cas9 in zebrafish. We show supernumerary guanine nucleotides at the 5’ ends of sgRNAs account for diminished CRISPR/Cas9 activity in zebrafish embryos. Genomic sequences can be targeted consistently with extremely high efficiency using Cas9 RNPs containing...
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| Publicado no: | Dev Cell |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6891219/ https://ncbi.nlm.nih.gov/pubmed/31708433 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.devcel.2019.10.004 |
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