Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology is effective for genome editing and now widely used in life science research. However, the key factors determining its editing efficiency and off-target cleavage activity for single-guide RNA (sgRNA) are poorly docume...

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Veröffentlicht in:Int J Biol Sci
Hauptverfasser: Zhao, Changzhi, Wang, Yunlong, Nie, Xiongwei, Han, Xiaosong, Liu, Hailong, Li, Guanglei, Yang, Gaojuan, Ruan, Jinxue, Ma, Yunlong, Li, Xinyun, Cheng, Huijun, Zhao, Shuhong, Fang, Yaping, Xie, Shengsong
Format: Artigo
Sprache:Inglês
Veröffentlicht: Ivyspring International Publisher 2019
Schlagworte:
Research Paper
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6854370/
https://ncbi.nlm.nih.gov/pubmed/31754336
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7150/ijbs.37152
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