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The Open Conformation Satisfies Multiple NMR Experiments on Apo Glutamine-Binding Protein: Overcoming Pitfalls in the Study of Interdomain Dynamics

Glutamine-binding protein (GlnBP) from Escherichia coli is a prototypical periplasmic binding protein that has been crystallized in an apo, “open” conformation, with its two domains far apart, and a holo, “closed” form, with proximal domains that engulf the cognate L-Gln ligand. A fundamental questi...

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Détails bibliographiques
Publié dans:Angew Chem Int Ed Engl
Auteurs principaux: Kooshapur, Hamed, Ma, Junhe, Tjandra, Nico, Bermejo, Guillermo A.
Format: Artigo
Langue:Inglês
Publié: 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6848765/
https://ncbi.nlm.nih.gov/pubmed/31515908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201911015
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