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Determination of the Solution Bound Conformation of an Amino-acid Binding Protein by NMR Paramagnetic Relaxation Enhancement: Use of a Single Flexible Paramagnetic Probe with Improved Estimation of Its Sampling Space

We demonstrate the feasibility of elucidating the bound (“closed”) conformation of a periplasmic binding protein, the glutamine-binding protein (GlnBP), in solution, using paramagnetic relaxation enhancements (PREs) arising from a single paramagnetic group. GlnBP consists of two globular domains con...

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Bibliografiske detaljer
Main Authors: Bermejo, Guillermo A., Strub, Marie-Paule, Ho, Chien, Tjandra, Nico
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2720827/
https://ncbi.nlm.nih.gov/pubmed/19583434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja902436g
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