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Determination of the Solution Bound Conformation of an Amino-acid Binding Protein by NMR Paramagnetic Relaxation Enhancement: Use of a Single Flexible Paramagnetic Probe with Improved Estimation of Its Sampling Space
We demonstrate the feasibility of elucidating the bound (“closed”) conformation of a periplasmic binding protein, the glutamine-binding protein (GlnBP), in solution, using paramagnetic relaxation enhancements (PREs) arising from a single paramagnetic group. GlnBP consists of two globular domains con...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2720827/ https://ncbi.nlm.nih.gov/pubmed/19583434 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja902436g |
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