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GENE-10. A SIMPLE AND EFFICIENT METHOD FOR GENERATING MULTI-ALLELIC INSERTION-DELETION MUTATIONS AND PRECISE DELETIONS IN PRIMARY HUMAN CELL CULTURES

CRISPR-Cas9-based technologies have revolutionized experimental manipulation of the human genome by enabling generation of site-specific genetic alterations, such as insertion-deletion (indel) mutations. None-the-less, limitations of the delivery and efficacy of these technologies restrict their app...

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Detalhes bibliográficos
Publicado no:Neuro Oncol
Main Authors: Hoellerbauer, Pia, Kufeld, Megan, Arora, Sonali, Wu, Hua-Jun, Feldman, Heather, Paddison, Patrick
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6847398/
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/neuonc/noz175.412
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