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Single Turnover RING/U-box E3-mediated Lysine Discharge Assays
RING and U-box ubiquitin ligases promote ubiquitin (Ub) transfer by priming Ub-conjugated E2 in a closed conformation to optimize the thioester bond for nucleophilic attack by substrate lysine. Here, we describe a single-turnover lysine discharge assay for direct assessment of the activity of any RI...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6847041/ https://ncbi.nlm.nih.gov/pubmed/30242700 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8706-1_2 |
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