CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci

Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Mao, Shiqi, Ying, Yachen, Wu, Xiaotian, Krueger, Christopher J, Chen, Antony K
Format: Artigo
Language:Inglês
Published: Oxford University Press 2019
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Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6847002/
https://ncbi.nlm.nih.gov/pubmed/31504824
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz752
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