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CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci
Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6847002/ https://ncbi.nlm.nih.gov/pubmed/31504824 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz752 |
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