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Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing

CRISPR/Cas9 is a powerful tool for genome editing in cells and organisms. Nevertheless, introducing directed templated changes by homology-directed repair (HDR) requires the cellular DNA repair machinery, such as the MRN complex (Mre11/Rad50/Nbs1). To improve the process, we tailored chimeric constr...

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Vydáno v:Biomolecules
Hlavní autoři: Reuven, Nina, Adler, Julia, Broennimann, Karin, Myers, Nadav, Shaul, Yosef
Médium: Artigo
Jazyk:Inglês
Vydáno: MDPI 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6843829/
https://ncbi.nlm.nih.gov/pubmed/31597252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/biom9100584
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