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An optimized molecular method for detection of influenza A virus using improved generic primers and concentration of the viral genomic RNA and nucleoprotein complex

For reported primer sets used to detect influenza A viruses (IAVs), we verified the nucleotide identities with 9,103 complete sequences of matrix (M) genes. At best, only 93.2% and 85.3% of the sequences had a 100% match with reported forward and reverse primers, respectively. Therefore, we designed...

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Bibliografiske detaljer
Udgivet i:J Vet Diagn Invest
Main Authors: Kim, Ji-Woon, Lee, Chung-Young, Nguyen, Thanh Trung, Kim, Il-Hwan, Kwon, Hyuk-Joon, Kim, Jae-Hong
Format: Artigo
Sprog:Inglês
Udgivet: SAGE Publications 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6838833/
https://ncbi.nlm.nih.gov/pubmed/30795722
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1040638719830760
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