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Single-Molecule Observation of Ligand Binding and Conformational Changes in FeuA

The specific binding of ligands by proteins and the coupling of this process to conformational changes is fundamental to protein function. We designed a fluorescence-based single-molecule assay and data analysis procedure that allows the simultaneous real-time observation of ligand binding and confo...

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Detalhes bibliográficos
Publicado no:Biophys J
Main Authors: de Boer, Marijn, Gouridis, Giorgos, Muthahari, Yusran Abdillah, Cordes, Thorben
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6838762/
https://ncbi.nlm.nih.gov/pubmed/31537314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2019.08.005
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