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Single-Molecule Observation of Ligand Binding and Conformational Changes in FeuA

The specific binding of ligands by proteins and the coupling of this process to conformational changes is fundamental to protein function. We designed a fluorescence-based single-molecule assay and data analysis procedure that allows the simultaneous real-time observation of ligand binding and confo...

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Publicat a:Biophys J
Autors principals: de Boer, Marijn, Gouridis, Giorgos, Muthahari, Yusran Abdillah, Cordes, Thorben
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6838762/
https://ncbi.nlm.nih.gov/pubmed/31537314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2019.08.005
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