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Using CAPTURE to detect spacer acquisition in native CRISPR arrays

CRISPR-Cas systems are able to acquire immunological memories (spacers) from bacteriophages and plasmids in order to survive infection, however this often occurs at low frequency within a population making it difficult to detect. Here we have developed CAPTURE (CRISPR Adaptation PCR Technique Using...

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Publicat a:Nat Protoc
Autors principals: McKenzie, Rebecca E., Almendros, Cristóbal, Vink, Jochem N.A., Brouns, Stan J.J.
Format: Artigo
Idioma:Inglês
Publicat: 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6831484/
https://ncbi.nlm.nih.gov/pubmed/30742049
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-018-0123-5
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