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Using CAPTURE to detect spacer acquisition in native CRISPR arrays
CRISPR-Cas systems are able to acquire immunological memories (spacers) from bacteriophages and plasmids in order to survive infection, however this often occurs at low frequency within a population making it difficult to detect. Here we have developed CAPTURE (CRISPR Adaptation PCR Technique Using...
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| Опубликовано в: : | Nat Protoc |
|---|---|
| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2019
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6831484/ https://ncbi.nlm.nih.gov/pubmed/30742049 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-018-0123-5 |
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