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Enhanced transgene expression using two β-globin MARs flanking expression cassettes in stably transfected CHO-K1 cells
In this study, we systemically investigated the positions and orientations of matrix attachment regions (MARs) in expression vectors to fully explore the mechanism for improving transgene expression. We constructed 14 vectors that incorporated human β-globin MARs into pIRES-eGFP backbone vectors. Th...
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| Pubblicato in: | 3 Biotech |
|---|---|
| Autori principali: | , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Springer International Publishing
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6825625/ https://ncbi.nlm.nih.gov/pubmed/31696040 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-019-1971-6 |
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