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Enhanced transgene expression using two β-globin MARs flanking expression cassettes in stably transfected CHO-K1 cells

In this study, we systemically investigated the positions and orientations of matrix attachment regions (MARs) in expression vectors to fully explore the mechanism for improving transgene expression. We constructed 14 vectors that incorporated human β-globin MARs into pIRES-eGFP backbone vectors. Th...

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Dades bibliogràfiques
Publicat a:3 Biotech
Autors principals: Zhang, Jihong, Zhang, Junhe, Cheng, Shan, Yang, Wenwen, Li, Shijiang
Format: Artigo
Idioma:Inglês
Publicat: Springer International Publishing 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6825625/
https://ncbi.nlm.nih.gov/pubmed/31696040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-019-1971-6
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