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Enhanced transgene expression using two β-globin MARs flanking expression cassettes in stably transfected CHO-K1 cells

In this study, we systemically investigated the positions and orientations of matrix attachment regions (MARs) in expression vectors to fully explore the mechanism for improving transgene expression. We constructed 14 vectors that incorporated human β-globin MARs into pIRES-eGFP backbone vectors. Th...

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Detalhes bibliográficos
Publicado no:3 Biotech
Main Authors: Zhang, Jihong, Zhang, Junhe, Cheng, Shan, Yang, Wenwen, Li, Shijiang
Formato: Artigo
Idioma:Inglês
Publicado em: Springer International Publishing 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6825625/
https://ncbi.nlm.nih.gov/pubmed/31696040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-019-1971-6
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