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Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Human 3-dimensional (3D) enteroid or colonoid cultures derived from crypt base stem cells are currently the most advanced ex vivo model of the intestinal epithelium. Due to their closed structures and significant supporting extracellular matrix, 3D cultures are not ideal for host-pathogen studies. E...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: In, Julie G., Foulke-Abel, Jennifer, Clarke, Elizabeth, Kovbasnjuk, Olga
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6816237/
https://ncbi.nlm.nih.gov/pubmed/31033964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59357
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