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Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Human 3-dimensional (3D) enteroid or colonoid cultures derived from crypt base stem cells are currently the most advanced ex vivo model of the intestinal epithelium. Due to their closed structures and significant supporting extracellular matrix, 3D cultures are not ideal for host-pathogen studies. E...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: In, Julie G., Foulke-Abel, Jennifer, Clarke, Elizabeth, Kovbasnjuk, Olga
Format: Artigo
Langue:Inglês
Publié: 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6816237/
https://ncbi.nlm.nih.gov/pubmed/31033964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59357
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