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Chlamydia DNA extraction for use in PCR: Stability and sensitivity in detection

We evaluated multiple procedures for extracting chlamydial DNA from specimens for detection in PCR tests. Commercial kits and an in‐house method were tested for their sensitivity and utility. Quantifiable chlamydial elementary bodies (EB) were used for spiking buffy coats from EDTA‐collected blood....

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Détails bibliographiques
Publié dans:J Clin Lab Anal
Auteurs principaux: Daugharty, H., Skelton, S. K., Messmer, T.
Format: Artigo
Langue:Inglês
Publié: Wiley Subscription Services, Inc., A Wiley Company 1998
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6807996/
https://ncbi.nlm.nih.gov/pubmed/9484669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/(SICI)1098-2825(1998)12:1<47::AID-JCLA8>3.0.CO;2-G
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