Application of a nested, multiplex PCR to psittacosis outbreaks.

We developed a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian specimens. The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present in clinical specimens. The target sequence was the 16S rRNA gene. The first-step PCR w...

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Detaylı Bibliyografya
Asıl Yazarlar: Messmer, T O, Skelton, S K, Moroney, J F, Daugharty, H, Fields, B S
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1997
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC229899/
https://ncbi.nlm.nih.gov/pubmed/9230378
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