Application of a nested, multiplex PCR to psittacosis outbreaks.

We developed a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian specimens. The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present in clinical specimens. The target sequence was the 16S rRNA gene. The first-step PCR w...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Messmer, T O, Skelton, S K, Moroney, J F, Daugharty, H, Fields, B S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229899/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9230378/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.8.2043-2046.1997
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