Application of a nested, multiplex PCR to psittacosis outbreaks.

We developed a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian specimens. The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present in clinical specimens. The target sequence was the 16S rRNA gene. The first-step PCR w...

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Vydáno v:	J Clin Microbiol
Hlavní autoři:	Messmer, T O, Skelton, S K, Moroney, J F, Daugharty, H, Fields, B S
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1997
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229899/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9230378/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.8.2043-2046.1997
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