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Application of a nested, multiplex PCR to psittacosis outbreaks.

We developed a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian specimens. The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present in clinical specimens. The target sequence was the 16S rRNA gene. The first-step PCR w...

詳細記述

保存先:
書誌詳細
出版年:J Clin Microbiol
主要な著者: Messmer, T O, Skelton, S K, Moroney, J F, Daugharty, H, Fields, B S
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology (ASM) 1997
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229899/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9230378/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.8.2043-2046.1997
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