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Detection of apoB‐100 R3500Q mutation by competitive allele‐specific polymerase chain reaction

The apolipoprotein B‐100 mutation R3500Q is one of the most common inherited defects causing abnormality of the lipid metabolism. We describe a one‐step, single‐tube PCR technique for detection of the mutation based on competition between allele‐specific primers. Three oligonucleotides are used: two...

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Bibliografske podrobnosti
izdano v:J Clin Lab Anal
Main Authors: Horvath, Anelia D., Kirov, Steven A., Karaulanov, Emil E., Ganev, Varban S.
Format: Artigo
Jezik:Inglês
Izdano: John Wiley & Sons, Inc. 2001
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6807702/
https://ncbi.nlm.nih.gov/pubmed/11574954
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcla.1037
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