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Detection of apoB‐100 R3500Q mutation by competitive allele‐specific polymerase chain reaction

The apolipoprotein B‐100 mutation R3500Q is one of the most common inherited defects causing abnormality of the lipid metabolism. We describe a one‐step, single‐tube PCR technique for detection of the mutation based on competition between allele‐specific primers. Three oligonucleotides are used: two...

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Detalhes bibliográficos
Publicado no:J Clin Lab Anal
Main Authors: Horvath, Anelia D., Kirov, Steven A., Karaulanov, Emil E., Ganev, Varban S.
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley & Sons, Inc. 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6807702/
https://ncbi.nlm.nih.gov/pubmed/11574954
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcla.1037
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